Review



dca  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    MedChemExpress dca
    Dca, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sodium+deoxycholate/Deoxycholic+acid+sodium+salt/pm42218181-193-3-12
    Average 95 stars, based on 62 article reviews
    dca - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Phosphorylation of PA28γ by CK2 kinase facilitates HNSCC tumor formation and progression.
    Article Snippet: .. 484 485 Immunoblot and immunoprecipitation analyses 486 Proteins were extracted from cells using RIPA buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% 487 NP-40, 0.5% sodium deoxycholate and 0.1% SDS) or NP-40 buffer (50 mM Tris (pH 7.4), 150 mM 488 NaCl and 1% NP-40) with protease inhibitor cocktail (Sigma, P8340) and phosphatase inhibitor 489 cocktail (MedChemExpress, HY-K0021). .. The cell lysates were clarified by centrifugation 490 (12000 g, 15 min, 4 °C), followed by protein quantification assays with the BCA protein assay kit 491 (Thermo Scientific, 23227) and the Varioskan LUX multimode microplate reader (Thermo 492 Scientific, VLB000GD1).

    Immunoprecipitation:

    Article Title: Phosphorylation of PA28γ by CK2 kinase facilitates HNSCC tumor formation and progression.
    Article Snippet: .. 484 485 Immunoblot and immunoprecipitation analyses 486 Proteins were extracted from cells using RIPA buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% 487 NP-40, 0.5% sodium deoxycholate and 0.1% SDS) or NP-40 buffer (50 mM Tris (pH 7.4), 150 mM 488 NaCl and 1% NP-40) with protease inhibitor cocktail (Sigma, P8340) and phosphatase inhibitor 489 cocktail (MedChemExpress, HY-K0021). .. The cell lysates were clarified by centrifugation 490 (12000 g, 15 min, 4 °C), followed by protein quantification assays with the BCA protein assay kit 491 (Thermo Scientific, 23227) and the Varioskan LUX multimode microplate reader (Thermo 492 Scientific, VLB000GD1).

    Article Title: Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication
    Article Snippet: DNA sequencing confirmed the expected specific sequence in the construct (Qingke, Xi'an, China). .. For immunoprecipitation analysis, the HEK293T cells were homogenized with IP lysis buffer (containing 50 mM Tris-Cl [pH = 7.4], 150 mM NaCl, 1%Triton-100, 0.1% SDS, 1% sodium deoxycholate, and protease inhibitor cocktail), centrifuged to remove cell debris, and then the supernatant was collected for immunoprecipitation with Myc (HY-K0206, MCE) and DYKDDDDK magnetic beads (b26103; Selleck Chemicals, USA) at 4°C for 2 h. After washing five times with IP lysis buffer, the protein-bound beads were finally resuspended in SDS-PAGE loading buffer. ..

    Protease Inhibitor:

    Article Title: Phosphorylation of PA28γ by CK2 kinase facilitates HNSCC tumor formation and progression.
    Article Snippet: .. 484 485 Immunoblot and immunoprecipitation analyses 486 Proteins were extracted from cells using RIPA buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% 487 NP-40, 0.5% sodium deoxycholate and 0.1% SDS) or NP-40 buffer (50 mM Tris (pH 7.4), 150 mM 488 NaCl and 1% NP-40) with protease inhibitor cocktail (Sigma, P8340) and phosphatase inhibitor 489 cocktail (MedChemExpress, HY-K0021). .. The cell lysates were clarified by centrifugation 490 (12000 g, 15 min, 4 °C), followed by protein quantification assays with the BCA protein assay kit 491 (Thermo Scientific, 23227) and the Varioskan LUX multimode microplate reader (Thermo 492 Scientific, VLB000GD1).

    Article Title: Lysine acetylation of PABPC1 C-terminal domain facilitates competitive recruitment of translation termination factor eRF3a
    Article Snippet: Flow-through anti-PTM antiserum was then affinity purified using either SLRS(AcK)VD or SLRS(Me2K)VD modified peptides (CovalAb). .. Cells were lysed in modified RIPA buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Igepal CA630, 0.2 % SDS, 10 mM sodium pyrophosphate, 25 mM 2-glycerophosphate, 0.5 % sodium deoxycholate, 10 mM sodium orthovanadate, 5 mM sodium fluoride, and 2 mM DTT] containing 1x Complete protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail 1 (Sigma) and 1x lysine deacetylase inhibitor cocktail (MedChemExpress). ..

    Article Title: Discovery of novel HDAC6 inhibitors based on the diphenyl-1,2,4-oxadiazole scaffold with potential efficacy in alleviating inflammation- and chemotherapy- associated mechanical hypersensitivity.
    Article Snippet: Current treatments for pain often have moderate efficacy and cause unwanted effects, highlighting the urgent need to develop more effective therapeutic strategies.. Accumulating evidence suggests that histone acetylation plays essential roles in acute and chronic pain.. Pharmacological inhibition of HDAC6 has emerged as a promising therapeutic approach for pain management.

    Article Title: Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication
    Article Snippet: DNA sequencing confirmed the expected specific sequence in the construct (Qingke, Xi'an, China). .. For immunoprecipitation analysis, the HEK293T cells were homogenized with IP lysis buffer (containing 50 mM Tris-Cl [pH = 7.4], 150 mM NaCl, 1%Triton-100, 0.1% SDS, 1% sodium deoxycholate, and protease inhibitor cocktail), centrifuged to remove cell debris, and then the supernatant was collected for immunoprecipitation with Myc (HY-K0206, MCE) and DYKDDDDK magnetic beads (b26103; Selleck Chemicals, USA) at 4°C for 2 h. After washing five times with IP lysis buffer, the protein-bound beads were finally resuspended in SDS-PAGE loading buffer. ..

    Article Title: Discovery of a Genetic Toxin-Antidote System in Vertebrates
    Article Snippet: .. To extract protein from dissected tissues, 50 mg of each sample were homogenized using RIPA buffer (150 mM NaCl (Millipore, cat# 116224), 0.5% Sodium deoxycholate (Biochemica, cat# A1531), and 50 mM Tris, pH 8.0 (KD Medical, cat# RGF-3360), and 1% Triton X-100) supplemented with the protease inhibitor cocktail (MedChem Express, cat# HY-K0010). .. Extracts were mixed with Laemmli buffer (BioRad, cat# 1610737) containing 2-mercaptoethanol (VWR Life Sciences, cat# 97064-590) and denatured at 95°C for 5 min.

    other:

    Article Title: CRISPR activation screens identify oncogenic lncRNAs that are susceptible to CDK4/6 inhibitor treatment.
    Article Snippet: Approximately 2 × 107 cells were harvested, washed once with PBS, and crosslinked with 0.3% formaldehyde (diluted with 16% formaldehyde, ThermoFisher, 28906) for 10 min at room temperature with rotation.

    Modification:

    Article Title: Lysine acetylation of PABPC1 C-terminal domain facilitates competitive recruitment of translation termination factor eRF3a
    Article Snippet: Flow-through anti-PTM antiserum was then affinity purified using either SLRS(AcK)VD or SLRS(Me2K)VD modified peptides (CovalAb). .. Cells were lysed in modified RIPA buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Igepal CA630, 0.2 % SDS, 10 mM sodium pyrophosphate, 25 mM 2-glycerophosphate, 0.5 % sodium deoxycholate, 10 mM sodium orthovanadate, 5 mM sodium fluoride, and 2 mM DTT] containing 1x Complete protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail 1 (Sigma) and 1x lysine deacetylase inhibitor cocktail (MedChemExpress). ..

    Histone Deacetylase Assay:

    Article Title: Lysine acetylation of PABPC1 C-terminal domain facilitates competitive recruitment of translation termination factor eRF3a
    Article Snippet: Flow-through anti-PTM antiserum was then affinity purified using either SLRS(AcK)VD or SLRS(Me2K)VD modified peptides (CovalAb). .. Cells were lysed in modified RIPA buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Igepal CA630, 0.2 % SDS, 10 mM sodium pyrophosphate, 25 mM 2-glycerophosphate, 0.5 % sodium deoxycholate, 10 mM sodium orthovanadate, 5 mM sodium fluoride, and 2 mM DTT] containing 1x Complete protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail 1 (Sigma) and 1x lysine deacetylase inhibitor cocktail (MedChemExpress). ..

    Lysis:

    Article Title: Discovery of novel HDAC6 inhibitors based on the diphenyl-1,2,4-oxadiazole scaffold with potential efficacy in alleviating inflammation- and chemotherapy- associated mechanical hypersensitivity.
    Article Snippet: Current treatments for pain often have moderate efficacy and cause unwanted effects, highlighting the urgent need to develop more effective therapeutic strategies.. Accumulating evidence suggests that histone acetylation plays essential roles in acute and chronic pain.. Pharmacological inhibition of HDAC6 has emerged as a promising therapeutic approach for pain management.

    Article Title: Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication
    Article Snippet: DNA sequencing confirmed the expected specific sequence in the construct (Qingke, Xi'an, China). .. For immunoprecipitation analysis, the HEK293T cells were homogenized with IP lysis buffer (containing 50 mM Tris-Cl [pH = 7.4], 150 mM NaCl, 1%Triton-100, 0.1% SDS, 1% sodium deoxycholate, and protease inhibitor cocktail), centrifuged to remove cell debris, and then the supernatant was collected for immunoprecipitation with Myc (HY-K0206, MCE) and DYKDDDDK magnetic beads (b26103; Selleck Chemicals, USA) at 4°C for 2 h. After washing five times with IP lysis buffer, the protein-bound beads were finally resuspended in SDS-PAGE loading buffer. ..

    Magnetic Beads:

    Article Title: Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication
    Article Snippet: DNA sequencing confirmed the expected specific sequence in the construct (Qingke, Xi'an, China). .. For immunoprecipitation analysis, the HEK293T cells were homogenized with IP lysis buffer (containing 50 mM Tris-Cl [pH = 7.4], 150 mM NaCl, 1%Triton-100, 0.1% SDS, 1% sodium deoxycholate, and protease inhibitor cocktail), centrifuged to remove cell debris, and then the supernatant was collected for immunoprecipitation with Myc (HY-K0206, MCE) and DYKDDDDK magnetic beads (b26103; Selleck Chemicals, USA) at 4°C for 2 h. After washing five times with IP lysis buffer, the protein-bound beads were finally resuspended in SDS-PAGE loading buffer. ..

    SDS Page:

    Article Title: Quantitative proteomic analysis identifies the unfolded protein response as a host pathway co-opted by ASFV to promote replication
    Article Snippet: DNA sequencing confirmed the expected specific sequence in the construct (Qingke, Xi'an, China). .. For immunoprecipitation analysis, the HEK293T cells were homogenized with IP lysis buffer (containing 50 mM Tris-Cl [pH = 7.4], 150 mM NaCl, 1%Triton-100, 0.1% SDS, 1% sodium deoxycholate, and protease inhibitor cocktail), centrifuged to remove cell debris, and then the supernatant was collected for immunoprecipitation with Myc (HY-K0206, MCE) and DYKDDDDK magnetic beads (b26103; Selleck Chemicals, USA) at 4°C for 2 h. After washing five times with IP lysis buffer, the protein-bound beads were finally resuspended in SDS-PAGE loading buffer. ..



    Similar Products

    95
    Thermo Fisher sodium deoxycholate
    Sodium Deoxycholate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sodium+deoxycholate/Sodium+deoxycholate+monohydrate%2C+97%25/pm42272181-96-9-11
    Average 95 stars, based on 1 article reviews
    sodium deoxycholate - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Shanghai Macklin Biochemical sodium deoxycholate nadc
    Sodium Deoxycholate Nadc, supplied by Shanghai Macklin Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sodium+deoxycholate/deoxycholate+sodium/pm42237357-272-5-29
    Average 86 stars, based on 1 article reviews
    sodium deoxycholate nadc - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    MedChemExpress dca
    Dca, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sodium+deoxycholate/Deoxycholic+acid+sodium+salt/pm42218181-193-3-12
    Average 95 stars, based on 1 article reviews
    dca - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Thermo Fisher sodium deoxycholate solution
    Sodium Deoxycholate Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sodium+deoxycholate/Sodium+deoxycholate+monohydrate%2C+97%25/bio_rxiv__64898__2026__05__10__723473-72-14-47
    Average 95 stars, based on 1 article reviews
    sodium deoxycholate solution - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Macklin Inc sodium deoxycholate
    Sodium Deoxycholate, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sodium+deoxycholate/deoxycholate+sodium/pm42066698-47-0-14
    Average 86 stars, based on 1 article reviews
    sodium deoxycholate - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results